Medimmune Sample Purification Robot
The sample purification robot was installed at Medimmune, Cambridge in 2007 and was designed to replace an existing system that required a lot of user intervention during the purification process. The robot is used as part of the development of human monoclonal antibody therapeutics and performs the initial sample purification by passing a number of reagents through SPE columns followed by the sample. The resultant purified sample is collected in Eppendorf tubes ready for aliquoting. The final samples are aliquoted into Micronics tubes, microtitre plates and PCR well plates. The robot runs 3 batches of 48 samples over a 24 hour period.
Main Features
- Capacity for 144 50ml Falcon input tubes
- Capacity for 10 reagents in 20 litre containers
- 6 sets of various size SPE columns (1ml, 3ml and 5ml) are stored in the robot
- 2 sets of syringes (10ml and 50ml) are automatically used depending on the required dilution accuracies
- Automated filtration and diluent waste management
- Integration with the Medimmune LIMS for input sample information and output sample identification for offline analysis
- Fully configurable dilution volumes and diluent selection for each stage of the purification process
